Tottenham’s record European goal scorer is ruled out of tonight’s return leg with Dnipro with a hamstring problem.
The striker will therefore bid farewell to the fans at half time, before he jets off to secure his move to MLS side Toronto.
Defoe will leave White Hart Lane with an impressive record of 91 goals for the club over two spells.
Boss Tim Sherwood said: “Jermain Defoe is possibly – along with Jimmy Greaves – one of the best goal-scorers this club has ever seen.
He has managed just one league goal from open play all season, since completing his big money switch from Valencia last summer.