Argentinos Juniors and America are through to the second phase of the Copa Libertadores after they both won 1-0 in Group three clashes on Wednesday.
Franco Niell scored the only goal for Argentinos in the 21st minute as they relegated Nacional to bottom spot.
But the win could prove costly for Argentinos as midfielders Sergio Escudero and Matias Laba, substitute Ciro Rais and Niell were all booked.
Argentinos finished top with seven points, one ahead of America who beat Fluminese thanks to a goal from defender Daniel Palacios in the 70th minute.
Deportes Tolima and Cruzeiro both qualified for the next phase after drawing 0-0. Cruzeiro finished top of Group seven on seven points three ahead of Deportes.
An injury-time penalty by Roberto Nanni helped Cerro Porteno snatch a point and retain top spot in Group five with a 1-1 draw against Santos.
The Brazilian side had taken the lead from the penalty spot in the 54th minute through Elanos.
Cerro have four points from one win and a draw while Santos have drawn both their matches.
San Luis and Once Caldas drew 1-1 in their Group one clash but the result was of little use to either side.
Renteria gave Once Caldas the lead after just two minutes but Michael Mina equalised for San Luis from a free kick in the 17th minute.
Once Caldas finished second bottom on two points while San Luis were bottom with one.