Coach Carlos Alberto Parreira slammed the performance of referee Massimo Busacca after South Africa's hopes of qualifying for the last 16 were left hanging by a thread following a 3-0 defeat to Uruguay.
Wednesday's defeat in Pretoria has left the hosts needing to overcome France in their final Group A encounter next week to reach the knockout stages.
Diego Forlan scored twice in the game but his second, which came via a penalty after South Africa goalkeeper Itumeleng Khune was sent off for a professional foul, left Parreira frustrated and angry.
"I haven't seen it properly because there were some players in the way, (but) the players are really annoyed, very angry and very disappointed in the referee," he fumed.
"Myself and everyone else said it was the worst referring performance in this competition so far.
"He was very unfair against our team from the very beginning, the way he acted against our team.
"He was giving fouls that weren't fouls, giving yellows that weren't yellows and then he left the ground with a smile on his face.
"I hope we don't see his face again in this tournament because he doesn't deserve to be here."
Meanwhile, Parreira still believes that South Africa can avoid the ignominy of being the first hosts in World Cup history to fail to qualify for the second round of the competition.
"Our hopes are not over," he added. "We need to beat France, but we will have to be more aggressive. Everything will be decided in the last game."